A one-day bioinformatics certificate course was conducted by Dr. Lavanyasri, RA, Assistant Professor, Department of Biotechnology, Mercy College, Palakkad on 19.01.2024. The One-Day Bioinformatics Certificate Course for final-year biotechnology undergraduates proved essential in bridging the gap between biology and computer science. Emphasizing biological databases, the basics of bioinformatics, and its applications, the program underlined the growing importance of computational skills in modern biotechnology. Participants gained insights into the significance of databases like GenBank and NCBI and various other databases under different categories crucial for genomics and proteomics research. The course uniquely focused on the integration of bioinformatics with wet lab research, highlighting the synergy between computational tools and traditional experimental approaches. By exploring applications in drug discovery and systems biology, the program equipped participants with a holistic view of bioinformatics’ real-world impact. The certificate course emerges as a transformative experience, positioning participants with a well-rounded skill set for success in the dynamic field of biotechnology. Through this certificate course guidance and the comprehensive syllabus, graduates leave not only with a certificate but also a profound understanding of how bioinformatics enhances their potential in biotechnological research.